Dr Robert A. Kinzie III, Hawaii Institute of Marine Biology Dr John W. Runcie, Hawaii Institute of Marine Biology Unknown This project assesses the physiological status of selected macroalgae in Hawaii under various nutrient regimes, with the purpose of determining a suitable physiological test that can, with reasonable accuracy, define nutrient concentrations in the adjacent water column. The project is designed to test the utility of pulse amplitude modulated (PAM) fluorescence as a means of assessing physiological status. PAM fluorescence provides an estimate of photosynthetic efficiency and can be measured underwater in the field. This technique may be suitable for resource managers seeking to rapidly assess nutrient concentrations in the marine and aquatic environment. The data were taken from July 2001 - July 2002. Support to understanding coral reef growth and health in Hawaii. NOAA Supplemental:Entry_ID: UnknownSensor_Name: Pulse Amplitude Modulated (PAM) fluorometer Source_Name: manual Project_Campaign: Hawaii Coral Reef Initiative Originating_Center: University of Hawaii Storage_Medium: Excel, ASCII Online_size: 267 kilobytes Resource Description: NODC Accession Number 0000428 20010701 0800 20020731 1700 ground condition Complete None Planned -157.8317 -157.7867 21.4766 21.4 None coral reef PAM florescence water quality nutrients Macroalgal bioindicators CoRIS Discovery Thesaurus Numeric Data Sets > Water Quality CoRIS Theme Thesaurus EARTH SCIENCE > Biosphere > Zoology > Corals > Reef Monitoring and Assessment > In Situ Nutrients EARTH SCIENCE > Biosphere > Zoology > Corals > Reef Damage Assessment > Pollution EARTH SCIENCE > Oceans > Ocean Optics > Fluorescence EARTH SCIENCE > Oceans > Coastal Processes > Coral Reefs ISO 19115 Topic Category biota 002 None Central North Pacific Hawaii Oahu Kaneohe Bay patch reefs CoRIS Region MHI CoRIS Place Thesaurus OCEAN BASIN > Pacific Ocean > Central Pacific Ocean > Hawaiian Islands > Oahu Island > Oahu (21N157W0003) COUNTRY/TERRITORY > United States of America > Hawaii > Honolulu > Oahu (21N157W0003) OCEAN BASIN > Pacific Ocean > Central Pacific Ocean > Hawaiian Islands > Oahu Island > Kaneohe Bay (21N157W0004) COUNTRY/TERRITORY > United States of America > Hawaii > Honolulu > Kaneohe Bay (21N157W0004) None NOAA and NODC would appreciate recognition as the resource from which these data were obtained in any publications and/or other representations of these data. NOAA/National Oceanographic Data Center NODC User Services Group Leader Mailing and physical address

SSMC3 Fourth Floor

1315 East West Highway

Silver Spring Maryland 20910-3282 USA 301-713-3277 301-713-3302 services@nodc.noaa.gov 8:00 a.m. to 4:00 p.m. Monday Through Friday Phone/FAX/e-mail This research is funded by the National Oceanic and Atmospheric Administration, Coastal Ocean Program, under awards NA870A0381, NA960P0187, NA060A0388, and NA160A1449 to the University of Hawaii for the Hawaii Coral Reef Initiative Research Program. MS Excel 2000 See Process Description. none The chlorophyte macroalgae Dictyosphaeria cavernosa was collected at Coconut Island in Kaneohe Bay, Oahu, Hawaii,and acclimated for one week in flowing seawater at the Hawaii Institute of Marine Biology research station prior to deployment in the field. The algae were cleaned, weighed and deployed at 3-4 m depth at three patch reefs in KaneoheBay (North, Central and South Bay sites). At each site four treatments were set up, each with three replicate algal samples. Each treatment was positioned 10 m apart to minimise interference. The treatments were 1) no nutrient addition, 2) Nitrogen addition,3) Phosphorus addition, and 4) Nitrogen and Phosphorus addition. After one week in the field, light response curves were carried out in situ on algal samples early in the morning. Samples were removed in the dark and analysed for chlorophyll a and b content, CNP composition, 15N:14N and13C:12C ratios, and mass increase (growth). This was carried out monthly for 13 months. Data collection - Data are presented raw, except for light response curve data, of which final calculated parameter estimates are provided. Data analysis - Values of the light response curve parameters ETRmax and alpha were derived by non-linear curve-fitting techniques. Unknown Dr. John Runcie Hawaii Institute of Marine Biology School of Ocean and Earth Science and Technology University of Hawaii mailing

P.O. Box 1346

Kaneohe Hawaii 96744 USA 808-236-7477 runcie@hawaii.edu NOAA/NESDIS/National Oceanographic Data Center Data Access Group, User Services Team mailing and physical

SSMC-3 Fourth Floor

1315 East West Highway

Silver Spring MD 20910-3282 USA 301-713-3277 301-713-3302 services@nodc.noaa.gov 8am-5pm, Monday through Friday Downloadable Data NOAA makes no warranty regarding these data, expressed or implied, nor does the fact of distribution constitute such a warranty. NOAA and NODC cannot assume liability for any damages caused by any errors or omissions in these data, nor as a result of the failure of these data to function on a particular system. ASCII http://accession.nodc.noaa.gov/428 Prices vary depending on data set, output medium and ordering mechanism. A standard handling charge, with additional costs for special handling, may be added to the basic cost of the data. Prepayment by check, money order or bank card is required. Orders may be placed via fax, email, regular mail, telephone or via the NNDC Online Store. 20121207 20081009 20060801 Mr. Patrick C. Caldwell NOAA/NESDIS/NODC/NCDDC Hawaii/US Pacific Liaison mailing

1000 Pope Road, MSB 316

Dept. of Oceanography

University of Hawaii at Manoa

Honolulu Hawaii 96822 USA (808)-956-4105 (808) 956-2352 caldwell@hawaii.edu 8 AM to 5 PM weekdays check services@nodc.noaa.gov if not available FGDC Content Standard for Digital Geospatial Metadata FGDC-STD-001-1998 20081009081349 None 20010701 20020731 http://www.coris.noaa.gov/metadata/records/html/nodc_0000428.html 2307